Journal: Immunity

Article Title: Lung γδ T cells mediate protective responses during neonatal influenza infection that are associated with Type-2 immunity

doi: 10.1016/j.immuni.2018.07.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The stained cells were resuspended in RNAse inhibitor containing sort buffer (RNAsin, Promega, 200U/μl) and sorted by gating on γδTCR + CD3 + cells directly into a 384-well PCR plate that had been preloaded with 1μl of reverse transcription reaction mix [1X SuperScript VILO Reaction Mix (Thermo Fisher), SuperScript VILO enzyme and 1X0.1% NP40 (Thermo Fisher)] with a sorter (Model SY3200, Sony Biotech Synergy sorter, Sony Biotech, San Jose, CA).

Techniques: Control, Virus, Plasmid Preparation, Recombinant, Cell Stimulation, Protease Inhibitor, Blocking Assay, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Sequencing, Software

Journal: Cell reports

Article Title: A FLCN-TFE3 Feedback Loop Prevents Excessive Glycogenesis and Phagocyte Activation by Regulating Lysosome Activity

doi: 10.1016/j.celrep.2020.01.042

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SuperScript IV VILO Master Mix with ezDNase Enzyme , Thermo Fisher Scientific , Cat#11766050.

Techniques: Purification, Blocking Assay, Recombinant, Transfection, Flow Cytometry, Magnetic Beads, Lysis, Extraction, SYBR Green Assay, Staining, shRNA, Sequencing, Plasmid Preparation, Software